The study examined the effects of Biology practical work on academic performance of secondary school students in Ikere Local Government Area of Ekiti State, Nigeria. The sample for the study comprised of one hundred and twenty five (125) students drawn from the population of study using simple sampling techniques. The instruments for data collection in this study was questionnaire and were analyzed using mean and standard deviation. The results revealed that students understand better when they involve themselves in practical experiment, thus matching theory with practical. Biology practical activities, stimulate creating, curiosity and critical thinking, there is no clear different between female and male on academic achievement in biology. It is recommended that teachers should endeavor to organize practical work for students as frequently as possible (at least once a week) so as to develop in the students the practical skills required of them for final examination. Also students should be advised to take every biology practical work very seriously. Finally, laboratory technicians should be employed by school authorities to assist biology teacher(s) in the area of practical work so that workload, time management and frequent practical are realistic to enhance students’ achievement.