The study investigated the importance of instructional material on learning of physical and health education students in Junior Secondary Schools Ikere Local Government Area of Ekiti State, Nigeria. Data were collected from three hundred (300) students. The instrument for the study was a 12 item self-structured questionnaire. The sample size of three hundred (300) respondents was used for the study. The respondents were from the junior classes of public Secondary schools in Ikere Local government area of Ekiti State. A test-re-test was employed to ascertain the reliability of the instrument, using Pearson’s Product Moment Correlation (PPMC). A coefficient of 0.86 was obtained at 0.05 level of significant. The demographic data were analysed using descriptive statistics tools while Chi-square (X2) was used to test all the hypotheses at 0.05 alpha level significance. The findings revealed that there is a significant impact of availability of instructional materials on the learning abilities of students in Physical and Health Education and also there is significant impact of the use of instructional materials by the teachers on the learning abilities of students in Physical and Health and Education. Conclusion and appropriate recommendations were made.